In general, methods such as the bile solubility test, optochin susceptibility, and colony morphology have been the methods of choice for species differentiation 5, 6. pneumoniae from other mitis group streptococci. Before reaching the point of pneumococcal serotyping identification, it is important to correctly identify S. pneumoniae and Streptococcus pseudopneumoniae can be a very difficult task and often requires several different methods 4, 5. The detection and differentiation of the mitis group and in particular between the two species S. It is therefore essential to be able to identify S.
Invasive pneumococcal disease (IPD) is one of the most frequent types of bacteraemia and meningitis in infants globally as well as in Denmark 2, 3. Worldwide, Streptococcus pneumoniae (pneumococci) infections cause high morbidity and mortality among children and elderly 1. Combining the bile solubility test and the MALDI-TOF spectra results provide a correct identification of all S. pneumoniae from other Streptococcus species.
The bile solubility test based on spectrophotometric reading described in this study can differentiate S. pseudopneumoniae strains but misidentified 13 other mitis group strains. Visual spectra evaluation correctly identified all S. pseudopneumoniae but misidentified 16 other mitis group strains. MALDI-TOF score value identification identified correctly 46 S. mitis isolate generated an OD-value above 2.1).
pneumoniae from all but one other mitis group streptococci (one S. Applying a calculated optimal cut-off absorbance-value of 2.1, differentiated S. Eighty-four species verified mitis group isolates were subjected to our bile solubility test (which measures and calculates the differences of absorbance in the test tube containing 10% sodium deoxycholate versus a blank control tube, after incubation for 10 minutes at 36 ☌ using a spectrophotometer) and MALDI-TOF MS (both the standard result output and by visual spectra evaluation). pneumoniae from Streptococcus pseudopneumoniae. This study assesses a novel bile solubility test and MALDI-TOF for the differentiation of Streptococcus pneumoniae from other mitis group streptococci, including differentiation of S.
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